This paper analyses whether the implementation of business and human rights (BHR) frameworks in Colombia properly responds to the challenges posed by informal mining and gender-based violence and discrimination in the context of conflict and peacebuilding. The mining sector has been considered key in Colombia to promote economic growth, but it is also characterized by significant informality. Informal mining in Colombia has been linked to gender-based violence and discrimination. We contend that while informality has been identified as a substantial hurdle to the realization of human rights, BHR frameworks still fall short in addressing this aspect of business. By examining the specific measures Colombia has devised to implement BHR, including two National Action Plans on BHR, we demonstrate the urgency of addressing informal economies in BHR and to continue developing particular insights to properly protect, respect and remedy the human rights wrongs women experience in the context of informal mining.